Project

Part:BBa_M31270:Design

Designed by: Michael Oh   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-27)


M13.1: Refactored M13K07 Phage


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1293
    Illegal XbaI site found at 1729
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1293
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1293
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1293
    Illegal XbaI site found at 1729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1293
    Illegal XbaI site found at 1729
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Overlaps were eliminated through direct duplication of appropriate sequences. Homologous recombination possibilities were diminished by silent mutation of the first instance of duplicated codons. About half the duplicated codons were changed in this manner.


Source

The source DNA is bases 1-2435 from the M13K07 phage, which is bounded by the HpaI and BamHI restriction sites.

References